Journal: Clinical & Experimental Ophthalmology

Article Title: Inhibitory Effects of 3′,4′‐Dihydroxyflavonol in a Rabbit Model of Minimally Invasive Bleb Surgery With PreserFlo MicroShunt

doi: 10.1111/ceo.14578

Figure Lengend Snippet: CD45 expression in rabbit blebs 2 weeks post‐ MIGS with PFMS. (A) Quantitative data, by positive pixel count of total CD45‐positive bleb areas (analysed after logarithmic transformation [natural log, ln]), within the cross‐sectional bleb region following post‐MIGS treatment with vehicle, DiOHF or MMC. Vehicle, 0.1% DMSO; DiOHF, 10 μM; MMC, 0.4 mg/mL (One‐way ANOVA with Tukey's post hoc test, n = 5, * signifies p < 0.05, ** signifies p < 0.01. Data are presented as mean ± SD). (B–D) Representative images of blebs from operated rabbit eyes that received post‐MIGS treatment to the surgical site with either the vehicle, DiOHF or MMC. Arrows indicate CD45‐positive cells. Bleb areas which are densely packed with CD45‐positive cells are outlined with a dotted red line. CD45‐positive cells are stained brown, with nuclei counterstained with haematoxylin. Scale bar, 200 μm.

Article Snippet: Sections were then incubated overnight at 4°C after being stained with one of the following primary antibodies: CD45 (1:200 dilution with PBS; MCA808GA; Bio‐Rad, CA, USA); vimentin (1:200 dilution with PBS; M0725; Dako, CA, USA); alpha smooth muscle actin (αSMA) (1:200 dilution with PBS; M0851; Dako, CA, USA); endothelial cells or platelet endothelial cell adhesion molecule‐1 (CD31/PECAM‐1; M0823); 1:100 dilution with Dako EnVision Flex Antibody Diluent (DM830; Dako, CA, USA); 3‐nitrotyrosine (3‐NT, 1:100 dilution with PBS; ab7048; Abcam Cambridge, UK), identifying the presence of ROS; and mouse IgG1 antibody (1:50 dilution with PBS; X0931, Dako, CA, USA) as a negative control.

Techniques: Expressing, Transformation Assay, Staining

Journal: Nature Communications

Article Title: Dual asparagine-depriving nanoparticles against solid tumors

doi: 10.1038/s41467-025-60798-y

Figure Lengend Snippet: a In vivo fluorescence imaging of tumor-bearing mice 24 h after intravenous injection of M Cy5.5 As and M Cy5.5 AHAs ( n = 3 mice). The unit of the scale bar is ×10 9 [p/s/cm 2 /sr]/[µW/cm 2 ]. b Left: Confocal fluorescence imaging of tumor sections after administration of M Cy5.5 As and M Cy5.5 AHAs. The 4T1 tumor cells and immune cells were indicated as CD44 and CD45 positive cells (red fluorescence), respectively. M Cy5.5 As and M Cy5.5 AHAs were pseudo-colored with green fluorescence. DAPI (blue fluorescence) was used to label the nuclei. Scale bars: 15 µm. Right: Values of Pearson’s correlation coefficient between Cy5.5-incorporated NPs and 4T1 tumor cells or immune cells. Data are represented as mean ± SD ( n = 10 independent samples). P values were calculated using an unpaired two-tailed Student’s t test. c Average tumor growth curves. Data are represented as mean ± SD ( n = 6 mice). P values were calculated using a two-way ANOVA followed by Tukey’s multiple comparisons test. d Survival curves of the mice receiving indicated treatment ( n = 6 mice). P values were calculated using a log-rank (Mantel–Cox) test. e The Asn levels in tumor cells isolated from tumors on day 26 after indicated treatment. Data are represented as mean ± SD ( n = 3 independent samples). P values were calculated using a one-way ANOVA followed by Tukey’s post-hoc test. f Heatmap of mRNA levels of ASNS , PSAT1 , GPT2 , MTHFD2 , and SLC1A5 in tumor cells isolated from tumors after indicated treatment. g Left: representative H&E-stained sections of lungs from indicated groups. Metastatic tumors are indicated with black dotted circles. Scale bars: 100 μm. See also Supplementary Fig. for complete data. Right: the number of tumor foci in lungs after indicated treatment. Data are represented as mean ± SD ( n = 3 mice). P values were calculated using a one-way ANOVA followed by Tukey’s post-hoc test. h , i Expression levels of N-cadherin ( h ) and E-cadherin ( i ) in tumors after indicated treatments. Data are represented as mean ± SD ( n = 3 independent samples). P values were calculated using a one-way ANOVA followed by Tukey’s post-hoc test. Asn asparagine, Cy5.5 cyanine 5.5, Rot rotenone, ASNase L -asparaginase. Source data are provided as a Source Data file.

Article Snippet: Tumor sections were stained with either anti-CD44 rabbit polyclonal antibody (Servicebio, GB112054 ) or anti-CD45 rabbit polyclonal antibody (Servicebio, GB113885 ), and visualized using CLSM.

Techniques: In Vivo, Fluorescence, Imaging, Injection, Two Tailed Test, Isolation, Staining, Expressing

Journal: Nature Communications

Article Title: Dual asparagine-depriving nanoparticles against solid tumors

doi: 10.1038/s41467-025-60798-y

Figure Lengend Snippet: a Schematic illustration of the experiment design for post-surgical therapy. Fourteen days after tumor inoculation, primary tumors were removed. Two days later, mice were subjected to various treatments every 3 days for a total of eight doses. On day 75, mice with no sign of tumor relapse and metastasis were challenged with 4T1-Luc cells. b In vivo bioluminescence imaging of the mice after indicated treatment ( n = 6 mice). The unit of the scale bar is ×10 7 p/s/cm 2 /sr. c Tumor growth curves of individual mice after indicated treatment ( n = 6 mice). d Ex vivo bioluminescence images of major organs ( n = 3 mice). The unit of the scale bar is ×10 6 p/s/cm 2 /sr. e Survival curves of the mice receiving the indicated treatment ( n = 6 mice). P values were calculated using a log-rank (Mantel–Cox) test. f In vivo bioluminescence imaging of mice before (day 74) and after (day 75, day 95, and day 105) tumor rechallenge ( n = 5 mice). The unit of the scale bar is ×10 7 p/s/cm 2 /sr. g , h FACS analysis of CD4 + Tcm (gated on CD45 + CD4 + population) ( g ) and CD8 + Tcm cells (gated on CD45 + CD8 + population) ( h ) in splenocytes. Data are represented as mean ± SD ( n = 3 independent samples). P values were calculated using an unpaired two-tailed Student’s t test. Rot rotenone, ASNase L -asparaginase, He heart, Li liver, Sp spleen, Lu lung, Ki kidney, Tcm central memory T. Source data are provided as a Source Data file.

Article Snippet: Tumor sections were stained with either anti-CD44 rabbit polyclonal antibody (Servicebio, GB112054 ) or anti-CD45 rabbit polyclonal antibody (Servicebio, GB113885 ), and visualized using CLSM.

Techniques: In Vivo, Imaging, Ex Vivo, Two Tailed Test

Journal: Scientific reports

Article Title: Comparison of the effects of fractional microneedle radiofrequency and microneedling on modulating the senescent fibroblast milieu in aged skin.

doi: 10.1038/s41598-025-02545-3

Figure Lengend Snippet: Fig. 3. Changes in the cellular milieu in the dermis following MNRF treatment. (a) Quantification of the total cell number in the dermis after MN and MNRF treatments. (b) Representative images of immunofluorescence staining for the fibroblast marker, HSP47 (red), and DAPI (blue) in the skin treated with MN and MNRF. The graph on the right indicates the number of HSP47-positive dermal fibroblasts after MN and MNRF treatments (n = 25). (c) Representative images of immunofluorescence staining for the leukocyte marker, CD45 (green); and macrophage marker, CD68 (red); and DAPI (blue) in skin treated with MN and MNRF. The graphs on the right represent the number of CD45-positive leukocytes and CD68-positive macrophages in the dermis after MN and MNRF treatments (n = 25). Original magnification ×400. Statistical significance: **p < 0.01, ns indicates no significance.

Article Snippet: The samples were incubated overnight at 4 °C with the following primary antibodies: HSP47, 1:1000 (ab226052; Abcam, Cambridge, UK); CD45, 1:200 (13917, Cell Signaling Technology, Danvers, MA, USA); CD68, Scientific Reports | (2025) 15:18296 8| https://doi.org/10.1038/s41598-025-02545-3 1:200 (M0814, Dako); p16INK4A, prediluted (805–4713, Ventana Medical Systems, Oro Valley, AZ, USA); procollagen-1, 1:50 (SP1.D8; DSHB, Iowa City, IA, United States); elastin, 1:1000 (ab77804; Abcam); and ki-67, 1:1000 (ab16667; Abcam).

Techniques: Immunofluorescence, Staining, Marker